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Product Details Each lot of this antibody is quality control tested by immunohistochemical staining. L1-mediated axon outgrowth occurs via a homophilic binding mechanism.
The optimal working dilution should be determined for each specific assay condition.
WB: 00* IP: 0 IHC: 0** This antibody is effective in immunoblotting, immunoprecipitation, and immunohistochemistry.
*Predicted MW = 200-220 k D **Recommend using Bouin's fixative as outlined in the Kamiguchi et al. Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain. H Kamiguchi, KE Long, M Pendergast, AW Schaefer, I Rapoport, T Kirchhausen, V Lemmon.